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High Throughput Sequencing

High throughput DNA sequencing is available on site, thanks to an Illumina MiSeq sequencer hosted at the IMM Genomic and Transcriptomic facility managed by M. Yann Denis.
Together with the facility, we developed sequencing pipelines for various bacteria and bacteriophages as well as pipelines dedicated to global approaches such as RNA-seq, ChIP-seq and Tn-seq. DNA Libraries preparation benefits from the know-how and the equipment’s available at the facility such as robotized RNA extraction, nucleic acids characterization with a Qubit™ fluorimeter from Invitrogen and a TapeStation 4200 system from Agilent or precise and reproducible mechanical fragmentation of nucleic acids with a Covaris M220 sonifier. Genomic and transcriptomic analyses are also available at the facility by (RT)-qPCR (Bio-Rad CFX96 system) and by digital (RT)-PCR (Stilla 3-colors naica® system).
Lionel Bertaux is in charge of setting up and developing digital (RT)-PCR technology at LCB.